Nad+ Buffered | Research Grade | COA Verified

Description

Research area: Cellular redox metabolism and NAD-consuming enzymes

Product Description

NAD+ (nicotinamide adenine dinucleotide) is not a peptide but a ubiquitous small-molecule dinucleotide coenzyme composed of nicotinamide and adenine bases linked through two ribose sugars and a pyrophosphate bridge. It is central to cellular redox biochemistry, cycling between its oxidized (NAD+) and reduced (NADH) states to shuttle electrons through glycolysis, the tricarboxylic-acid cycle, and oxidative phosphorylation. Beyond redox chemistry, NAD+ also serves as a consumed substrate for several enzyme families rather than acting catalytically. This buffered research-grade preparation is formulated for solution stability, and NAD+ is widely used as a foundational reference cofactor in metabolic, mitochondrial, and cellular-aging research across in-vitro and preclinical model systems.

Mechanism & Research Context

In research models NAD+ functions both as an electron carrier in energy-yielding metabolic pathways and as a substrate for NAD-consuming enzymes. These include the sirtuin deacylases (SIRT1-7), which couple NAD+ hydrolysis to protein deacetylation; poly(ADP-ribose) polymerases (PARPs) involved in DNA-damage signaling; and CD38/CD157 glycohydrolases that generate calcium-mobilizing messengers. Studies manipulate NAD+ availability to probe how cellular redox balance and these enzyme activities influence mitochondrial function, gene regulation, and stress responses.

Research Applications

• In-vitro study of sirtuin (SIRT1-7) NAD+-dependent deacylation
• PARP-mediated DNA-damage-response signaling assays
• CD38/CD157 NAD+-hydrolase and calcium-signaling research
• Mitochondrial bioenergetics and NAD+/NADH redox-ratio studies
• Preclinical cellular-senescence and metabolic model systems

Storage & Handling

• Store sealed vials in a cool, dark place for short-term storage and frozen for long-term storage.
• After reconstitution, refrigerate and protect from light and heat.
• Avoid repeated freeze-thaw cycles; handle using standard laboratory safety and aseptic technique.

Product Specifications

• Compound class: Pyridine-dinucleotide redox coenzyme (small molecule)
• Form: Lyophilized powder
• Purity: ≥ 99% (HPLC verified)
• Appearance: White to off-white powder
• Third-party tested: Yes — Certificate of Analysis (COA) on this product page

Quality & Third-Party Testing

Every batch of Nad+ Buffered is independently third-party tested in the USA for identity and purity, with a Certificate of Analysis (COA) available on this product page.

Compliance Notice

Nad+ Buffered is for research use only. It is not intended for human or veterinary use and has not been evaluated by the FDA. By purchasing, the buyer represents and warrants that it will be used solely for in-vitro laboratory research.