PE-22-28 | Research Grade | COA Verified

Description

Research area: TREK-1 potassium channel and neurogenesis research

Product Description

PE-22-28 is a synthetic seven-amino-acid peptide developed in 2017 by Djillani and colleagues as a shortened, optimized analogue of spadin, a naturally occurring peptide cleaved from the propeptide of the sortilin/neurotensin-receptor-3 precursor. Compared with the parent spadin, PE-22-28 was engineered for markedly greater potency and improved in-vivo stability, with reported TREK-1 inhibition in the sub-nanomolar range and a substantially extended duration of action in animal studies. This makes it a valued laboratory tool for probing two-pore-domain potassium-channel biology. Within neuropharmacology research it is distinguished as one of the most potent spadin-derived TREK-1 blockers characterized to date in preclinical behavioral and cellular models.

Mechanism & Research Context

PE-22-28 is studied as a selective blocker of TREK-1 (TWIK-related potassium channel 1), a two-pore-domain background potassium channel expressed in brain regions implicated in mood regulation. Because TREK-1-knockout animals display a depression-resistant phenotype, the channel is a validated research target. In rodent models PE-22-28 inhibition of TREK-1 is investigated for downstream effects on neuronal excitability, hippocampal neurogenesis, and antidepressant-like behavioral endpoints observed within days of administration.

Research Applications

• In-vitro TREK-1 potassium-channel inhibition and electrophysiology assays
• Rodent forced-swim and novelty-suppressed-feeding behavioral models
• Study of hippocampal neurogenesis following channel blockade
• Structure-activity comparison against parent peptide spadin
• Investigation of two-pore-domain channel roles in mood-related circuits

Storage & Handling

• Store sealed vials in a cool, dark place for short-term storage and frozen for long-term storage.
• After reconstitution, refrigerate and protect from light and heat.
• Avoid repeated freeze-thaw cycles; handle using standard laboratory safety and aseptic technique.

Product Specifications

• Compound class: Synthetic spadin-analogue heptapeptide channel blocker
• Form: Lyophilized powder
• Purity: ≥ 99% (HPLC verified)
• Appearance: White to off-white powder
• Third-party tested: Yes — Certificate of Analysis (COA) on this product page

Quality & Third-Party Testing

Every batch of PE-22-28 is independently third-party tested in the USA for identity and purity, with a Certificate of Analysis (COA) available on this product page.

Compliance Notice

PE-22-28 is for research use only. It is not intended for human or veterinary use and has not been evaluated by the FDA. By purchasing, the buyer represents and warrants that it will be used solely for in-vitro laboratory research.