TB4 Thymosin Beta-4 (TB500) | Research Grade | COA Verified

Description

Research area: Cytoskeletal dynamics and tissue-repair research models

Product Description

TB-500 in its full-length form is synthetic Thymosin Beta-4, a 43-amino-acid naturally occurring polypeptide of the beta-thymosin family found abundantly in platelets, wound fluid, and most mammalian cell types. Weighing roughly 4.9 kDa, the intact protein carries the central actin-binding motif alongside additional structural domains that truncated derivatives lack. It is a G-actin-sequestering peptide rather than a small molecule, and its complete sequence supports functions in cytoskeletal remodeling, cell migration, and angiogenic signaling. In laboratory settings it is distinguished from short 17-23 fragments by its greater size, broader binding surface, and fuller repertoire of documented in-vitro activities across regeneration and inflammation models.

Mechanism & Research Context

The intact protein binds monomeric globular actin (G-actin) in a 1:1 complex, sequestering it and shifting the equilibrium between G-actin and filamentous F-actin. This regulation of cytoskeletal dynamics underlies observed effects on cell migration in scratch-wound assays. Full-length Thymosin Beta-4 is additionally studied for interactions influencing angiogenesis, laminin and integrin signaling, and downregulation of pro-inflammatory mediators in cultured cells and rodent tissue-injury models, where the complete sequence is compared against isolated actin-binding fragments.

Research Applications

• G-actin sequestration and cytoskeletal-dynamics assays
• Cell-migration and scratch-wound closure models in vitro
• Angiogenesis and endothelial tube-formation studies
• Cardiac and corneal tissue-repair investigations in animal models
• Comparative activity studies against truncated Tb4 fragments

Storage & Handling

• Store sealed vials in a cool, dark place for short-term storage and frozen for long-term storage.
• After reconstitution, refrigerate and protect from light and heat.
• Avoid repeated freeze-thaw cycles; handle using standard laboratory safety and aseptic technique.

Product Specifications

• Compound class: Full-length 43-amino-acid beta-thymosin polypeptide (actin-sequestering protein)
• Form: Lyophilized powder
• Purity: ≥ 99% (HPLC verified)
• Appearance: White to off-white powder
• Third-party tested: Yes — Certificate of Analysis (COA) on this product page

Quality & Third-Party Testing

Every batch of TB4 Thymosin Beta-4 (TB500) is independently third-party tested in the USA for identity and purity, with a Certificate of Analysis (COA) available on this product page.

Compliance Notice

TB4 Thymosin Beta-4 (TB500) is for research use only. It is not intended for human or veterinary use and has not been evaluated by the FDA. By purchasing, the buyer represents and warrants that it will be used solely for in-vitro laboratory research.