TB-500/TB4 FRAG 889Da (17-23) | Research Grade | COA Verified

Description

Research area: Minimal actin-binding motif and structure-activity research

Product Description

This product is the short synthetic fragment of Thymosin Beta-4 rather than the intact 43-amino-acid protein. Corresponding to residues 17-23, it centers on the acetylated actin-binding heptapeptide sequence LKKTETQ, with a molecular weight near 889 Da. This minimal motif represents the region of Tb4 most directly implicated in binding globular actin, isolated away from the larger protein’s additional domains. As a compact, low-molecular-weight peptide it is chemically and functionally distinct from full-length TB-500: it lacks the flanking sequences that contribute to angiogenic and integrin-related activities, and is studied specifically to probe the contribution of the core actin-binding region.

Mechanism & Research Context

The heptapeptide reproduces the central actin-binding determinant of Thymosin Beta-4, interacting with G-actin monomers to influence actin-polymerization equilibria in cell-free and cultured-cell systems. Research uses the isolated fragment to dissect which observed cytoskeletal and migratory effects depend on the core LKKTETQ motif versus the surrounding protein scaffold. In rodent injury models it is examined for actin-mediated contributions to cell motility, distinct from the broader signaling attributed to the complete protein.

Research Applications

• Mapping the minimal actin-binding motif of Tb4
• Structure-activity comparisons against full-length Thymosin Beta-4
• G-actin interaction and polymerization assays in vitro
• Cell-motility contribution studies in cultured cells
• Fragment-specific stability and handling investigations

Storage & Handling

• Store sealed vials in a cool, dark place for short-term storage and frozen for long-term storage.
• After reconstitution, refrigerate and protect from light and heat.
• Avoid repeated freeze-thaw cycles; handle using standard laboratory safety and aseptic technique.

Product Specifications

• Compound class: Short synthetic acetylated heptapeptide fragment of Thymosin Beta-4
• Form: Lyophilized powder
• Purity: ≥ 99% (HPLC verified)
• Appearance: White to off-white powder
• Third-party tested: Yes — Certificate of Analysis (COA) on this product page

Quality & Third-Party Testing

Every batch of TB-500/TB4 FRAG 889Da (17-23) is independently third-party tested in the USA for identity and purity, with a Certificate of Analysis (COA) available on this product page.

Compliance Notice

TB-500/TB4 FRAG 889Da (17-23) is for research use only. It is not intended for human or veterinary use and has not been evaluated by the FDA. By purchasing, the buyer represents and warrants that it will be used solely for in-vitro laboratory research.