5-Amino-1MQ | Research Grade | COA Verified

Description

Research area: NAD+ metabolism and NNMT enzyme research

Product Description

5-Amino-1MQ is not a peptide but a small-molecule aromatic heterocycle, specifically 5-amino-1-methylquinolinium, a low-molecular-weight quinolinium scaffold bearing an amino substituent. It was characterized by Neelakantan and colleagues (2018) as a potent, orally bioavailable, membrane-permeable inhibitor of nicotinamide N-methyltransferase (NNMT). This distinguishes it chemically from the peptide products typically stocked for research; it is a synthetic organic compound rather than an amino-acid chain. Its selectivity over related methyltransferases and its ability to enter adipocytes have made it a widely used chemical probe for interrogating NNMT biology and NAD+ dynamics in metabolic tissue and cellular models.

Mechanism & Research Context

In laboratory models it competitively binds the active site of NNMT, the enzyme that methylates nicotinamide into 1-methylnicotinamide while consuming the methyl donor S-adenosylmethionine. Inhibition preserves nicotinamide for recycling through the NAMPT-dependent NAD+ salvage pathway, raising intracellular NAD+ and downstream sirtuin (SIRT1) activity. Researchers apply it in cultured adipocytes and rodent metabolic-tissue studies to probe NNMT's role in cellular energy metabolism, mitochondrial function, and methyl-group flux.

Research Applications

• Chemical-probe inhibition of NNMT enzymatic activity in vitro
• NAD+ salvage-pathway and methyl-donor (SAM) flux studies
• Adipocyte energy-metabolism and mitochondrial function research
• Sirtuin (SIRT1) signaling investigations in cell models
• Structure-activity studies of quinolinium NNMT inhibitors

Storage & Handling

• Store sealed vials in a cool, dark place for short-term storage and frozen for long-term storage.
• After reconstitution, refrigerate and protect from light and heat.
• Avoid repeated freeze-thaw cycles; handle using standard laboratory safety and aseptic technique.

Product Specifications

• Compound class: Small-molecule NNMT inhibitor (1-methylquinolinium derivative)
• Form: Lyophilized powder
• Purity: ≥ 99% (HPLC verified)
• Appearance: White to off-white powder
• Third-party tested: Yes — Certificate of Analysis (COA) on this product page

Quality & Third-Party Testing

Every batch of 5-Amino-1MQ is independently third-party tested in the USA for identity and purity, with a Certificate of Analysis (COA) available on this product page.

Compliance Notice

5-Amino-1MQ is for research use only. It is not intended for human or veterinary use and has not been evaluated by the FDA. By purchasing, the buyer represents and warrants that it will be used solely for in-vitro laboratory research.